LmrS is a multidrug efflux pump of the major facilitator superfamily from Staphylococcus aureus.

LmrS is a multidrug efflux pump of the major facilitator superfamily from Staphylococcus aureus.

Antimicrobial agents and chemotherapy (2010-09-22)

Jody L Floyd, Kenneth P Smith, Sanath H Kumar, Jared T Floyd, Manuel F Varela

PMID20855745

ABSTRACT

A multidrug efflux pump designated LmrS (lincomycin resistance protein of Staphylococcus aureus), belonging to the major facilitator superfamily (MFS) of transporters, was cloned, and the role of LmrS in antimicrobial efflux was evaluated. The highest relative increase in MIC, 16-fold, was observed for linezolid and tetraphenylphosphonium chloride (TPCL), followed by an 8-fold increase for sodium dodecyl sulfate (SDS), trimethoprim, and chloramphenicol. LmrS has 14 predicted membrane-spanning domains and is homologous to putative lincomycin resistance proteins of Bacillus spp., Lactobacillus spp., and Listeria spp.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

L3771

Sigma-Aldrich

Sodio dodecil solfato, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

H5882

Sigma-Aldrich

Esadeciltrimetilammonio bromuro, ≥98%

H6269

Sigma-Aldrich

Esadeciltrimetilammonio bromuro, for molecular biology, ≥99%

71736

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Sodio dodecil solfato, BioUltra, for molecular biology, 10% in H₂O

R3501

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Rifampicin, ≥95% (HPLC), powder or crystals

436143

Sigma-Aldrich

Sodio dodecil solfato, ACS reagent, ≥99.0%

L4509

Sigma-Aldrich

Sodio dodecil solfato, ReagentPlus^®, ≥98.5% (GC)

H9151

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Esadeciltrimetilammonio bromuro, BioXtra, ≥99%

71725

Sigma-Aldrich

Sodio dodecil solfato, BioUltra, for molecular biology, ≥99.0% (GC)

C2759

Sigma-Aldrich

Carbonyl cyanide 3-chlorophenylhydrazone, ≥97% (TLC), powder

E5389

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Erythromycin, BioReagent, suitable for cell culture

T7883

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Trimethoprim, ≥98.5%

E7637

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Ethidium bromide, BioReagent, for molecular biology, powder

92131

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Trimethoprim, ≥99.0% (HPLC)

E1385

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Bromuro di etidio, BioReagent, for molecular biology, 500 μg/mL in H₂O

52365

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Esadeciltrimetilammonio bromuro, BioUltra, for molecular biology, ≥99.0% (AT)

L6026

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Sodio dodecil solfato, BioXtra, ≥99.0% (GC)

R7382

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Rifampicin, suitable for plant cell culture, BioReagent, ≥95% (HPLC), powder or crystals

71729

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Sodio dodecil solfato, ≥98.0% (GC)

L5750

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Sodio dodecil solfato, 92.5-100.5% based on total alkyl sulfate content basis

R0875

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Reserpine

71726

Supelco

Sodio dodecil solfato, suitable for ion pair chromatography, LiChropur^™, ≥99.0%

46067

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Bromuro di etidio, for fluorescence, ~1% in H₂O

218790

Sigma-Aldrich

Tetraphenylphosphonium chloride, 98%

218782

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Tetraphenylphosphonium bromide, 97%

71717

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Sodio dodecil solfato, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

E8751

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Ethidium bromide, ~95% (HPLC)

62862

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Sodio dodecil solfato, ≥90% ((Assay))

52370

Sigma-Aldrich

Esadeciltrimetilammonio bromuro, ≥96.0% (AT)

E6376

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Erythromycin, potency: ≥850 μg per mg