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ID30A-3 (MASSIF-3) - a beamline for macromolecular crystallography at the ESRF with a small intense beam.

Journal of synchrotron radiation (2020-05-10)
David von Stetten, Philippe Carpentier, David Flot, Antonia Beteva, Hugo Caserotto, Fabien Dobias, Matias Guijarro, Thierry Giraud, Mario Lentini, Sean McSweeney, Antoine Royant, Sebastien Petitdemange, Jeremy Sinoir, John Surr, Olof Svensson, Pascal Theveneau, Gordon A Leonard, Christoph Mueller-Dieckmann
ABSTRACT

ID30A-3 (or MASSIF-3) is a mini-focus (beam size 18 µm × 14 µm) highly intense (2.0 × 1013 photons s-1), fixed-energy (12.81 keV) beamline for macromolecular crystallography (MX) experiments at the European Synchrotron Radiation Facility (ESRF). MASSIF-3 is one of two fixed-energy beamlines sited on the first branch of the canted undulator setup on the ESRF ID30 port and is equipped with a MD2 micro-diffractometer, a Flex HCD sample changer, and an Eiger X 4M fast hybrid photon-counting detector. MASSIF-3 is recommended for collecting diffraction data from single small crystals (≤15 µm in one dimension) or for experiments using serial methods. The end-station has been in full user operation since December 2014, and here its current characteristics and capabilities are described.

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Sigma-Aldrich
Tripsina, powder, ≥7,500 BAEE units/mg solid