SRP0733

PCTK2 (CDK17)/CYCLINY, Active

PRECISIO^® Kinase, recombinant

• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Ricombinante: expressed in baculovirus infected Sf9 cells
• Saggio: 70-95% (SDS-PAGE)
• Forma fisica: frozen liquid
• Attività specifica: 15-25 nmol/min·mg (radiometric)
• PM: ~64 kDa (Cyclin Y); ~88 kDa (PCTK2)
• Concentrazione: 0.1 μg/μL
• Numero d’accesso: NM_002595; NM_145012
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... CCNY(219771) , CDK17(5128)

Descrizione

Applicazioni

Useful for kinase assay and Western blot.

Azioni biochim/fisiol

Recombinant full-length human PCTK2 (CDK17) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons ⁽¹⁾. The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell cycle together with the emerging large family of cyclins ⁽²⁾.

Ricostituzione

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Stoccaggio e stabilità

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Note legali

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable