SRP0149

Set7/Set9 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Sinonimo/i: KMT7, Lysine N-methyltransferase 7, SET domain containing (lysine methyltransferase) 7, SET7/9, SETD7
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Saggio: ≥70% (SDS-PAGE)
• Forma fisica: aqueous solution
• PM: 61 kDa
• Confezionamento: pkg of 100 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_030648
• N° accesso UniProt: Q8WTS6
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... SETD7(80854)

Descrizione

Descrizione generale

Human recombinant SET7/9, amino acids 52-end, with N-terminal GST tag, GenBank Accession No. NM_030648, expressed in a E. coli expression system. MW = 61 kDa,

SET7/9 also known as KMT7/SET domain containing lysine methyltransferase 7 (SETD7), is encoded by the gene mapped to human chromosome 4q31.1. The encoded protein belongs to SET domain-containing protein family.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Azioni biochim/fisiol

SET7/9 acts as a histone methyltransferase and catalyzes monomethylation of histone H3 at lysine 4 (H3K4me1) in vitro. Down-regulation of SET7/9 expression affects human embryonic stem cell differentiation. The encoded protein is implicated in the regulation of proteins and transcription factor activity. It also stimulates promoters of methylation-dependent co-recruitment through non-histone protein methylation. Mutation in the gene encoding SET7/9 is associated with serum lycopene concentrations. Elevated expression of the gene is associated with progression of hepatocellular carcinoma (HCC).

Definizione di unità

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable