Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

View All Documentation

R3400

Sigma-Aldrich

Monoclonal Anti-c-K-Ras antibody produced in mouse

clone 234-4.2, purified immunoglobulin, buffered aqueous solution

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Numero MDL:
MFCD00239650
Codice UNSPSC:
12352203

Origine biologica

mouse

Livello qualitativo

200

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

234-4.2, monoclonal

Forma fisica

buffered aqueous solution

PM

antigen 21 kDa

Reattività contro le specie

mouse, human, rat

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10 μg/mL using normal skin tissue
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2κ

N° accesso UniProt

P01116

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... KRAS(3845)
mouse ... Kras(16653)
rat ... Kras(24525)

Descrizione generale

c-K-Ras or KRAS (kirsten rat sarcoma-2 virus oncogene) is a small GTP-binding protein. It is located on human chromosome 12p12.1. KRAS has two splice variants, such as KRASA and KRASB (KRAS proto-oncogenes). Expression of KRASA is restricted to specific tissues whereas KRASB is expressed everywhere .

Specificità

The antibody reacts with c-K-Ras and v-K-Ras p21. It crossreacts with cH-Ras and cN-Ras p21 on immunoblots.

Immunogeno

recombinant p21 protein

Applicazioni

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Azioni biochim/fisiol

KRAS (kirsten rat sarcoma-2 virus oncogene) mutation leads to poor prognosis of early rectal cancer.

Stato fisico

Solution in 0.05 M sodium phosphate buffer, pH7.5, containing 0.2% gelatin and < 0.1% sodium azide

Nota sulla preparazione

Purified using protein A or G

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Non trovi il prodotto giusto?  

Prova il nostro Motore di ricerca dei prodotti.

Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

David A Iglesias et al.
Molecular cancer therapeutics, 12(12), 2847-2856 (2013-10-01)
Metformin is an oral biguanide commonly used for the treatment of type II diabetes and has recently been demonstrated to possess antiproliferative properties that can be exploited for the prevention and treatment of a variety of cancers. The mechanisms underlying
Jasminka Omerovic et al.
The Biochemical journal, 426(1), 65-72 (2009-11-20)
Oncogenic Ras mutations render the protein constitutively active and promote tumorigenesis via chronic stimulation of effector pathways. In A549 lung adenocarcinoma approx. 50% of the total Ras population is constitutively active, yet these cells display only weak activation of the
Candida Zuchegna et al.
Redox report : communications in free radical research, 27(1), 150-157 (2022-07-14)
Although the protooncogenes small GTPases Ras are redox-sensitive proteins, how they are regulated by redox signaling in the central nervous system (CNS) is still poorly understood. Alteration in redox-sensitive targets by redox signaling may have myriad effects on Ras stability
Harrison J Hocker et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(25), 10201-10206 (2013-06-06)
Aberrant signaling by oncogenic mutant rat sarcoma (Ras) proteins occurs in ∼15% of all human tumors, yet direct inhibition of Ras by small molecules has remained elusive. Recently, several small-molecule ligands have been discovered that directly bind Ras and inhibit
Kwang-jin Cho et al.
The Journal of biological chemistry, 287(52), 43573-43584 (2012-11-06)
Oncogenic mutant Ras is frequently expressed in human cancers, but no anti-Ras drugs have been developed. Since membrane association is essential for Ras biological activity, we developed a high content assay for inhibitors of Ras plasma membrane localization. We discovered
Visualizza tutti i documenti correlati

Articoli

esiRNA Knockdown Efficiency Tested by Western Blotting

Quantitative and qualitative western blotting to validate knockdown by esiRNA.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.