N1290
Polynucleotide phosphorylase human
Sinonimo/i:
Polyribonucleotide nucleotidyltransferase
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About This Item
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Ricombinante
expressed in E. coli
Forma fisica
solution
enzyme activity
≥20 units/mg protein
PM
~90 kDa
Concentrazione
400-600 μg/mL protein
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Azioni biochim/fisiol
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.
Stato fisico
supplied as a solution in 20 mM HEPES buffer, pH 7.9, with 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, ~130 mM KCl, and 20% (w/v) glycerol.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Lukasz S Borowski et al.
Biochimica et biophysica acta, 1797(6-7), 1066-1070 (2010-02-02)
Protein complexes responsible for RNA degradation play important role in three key aspects of RNA metabolism: they control stability of physiologically functional transcripts, remove the unnecessary RNA processing intermediates and destroy aberrantly formed RNAs. In mitochondria the post-transcriptional events seem
Robert N Rainey et al.
Molecular and cellular biology, 26(22), 8488-8497 (2006-09-13)
Polynucleotide phosphorylase (PNPase) is an exoribonuclease and poly(A) polymerase postulated to function in the cytosol and mitochondrial matrix. Prior overexpression studies resulted in PNPase localization to both the cytosol and mitochondria, concurrent with cytosolic RNA degradation and pleiotropic cellular effects
Hsiao-Wen Chen et al.
Molecular and cellular biology, 26(22), 8475-8487 (2006-09-13)
We recently identified polynucleotide phosphorylase (PNPase) as a potential binding partner for the TCL1 oncoprotein. Mammalian PNPase exhibits exoribonuclease and poly(A) polymerase activities, and PNPase overexpression inhibits cell growth, induces apoptosis, and stimulates proinflammatory cytokine production. A physiologic connection for
Victoria Portnoy et al.
RNA (New York, N.Y.), 14(2), 297-309 (2007-12-18)
PNPase is a major exoribonuclease that plays an important role in the degradation, processing, and polyadenylation of RNA in prokaryotes and organelles. This phosphorolytic processive enzyme uses inorganic phosphate and nucleotide diphosphate for degradation and polymerization activities, respectively. Its structure
Takashi Nagaike et al.
The Journal of biological chemistry, 280(20), 19721-19727 (2005-03-17)
Mammalian mitochondrial (mt) mRNAs have short poly(A) tails at their 3' termini that are post-transcriptionally synthesized by mt poly(A) polymerase (PAP). The polyadenylation of mt mRNAs is known to be a key process needed to create UAA stop codons that
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