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MAK417

Sigma-Aldrich

Glycogen Phosphorylase Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Codice UNSPSC:
12161503

Metodo di rivelazione

colorimetric

Malattie correlate

cancer

Temperatura di conservazione

−20°C

Descrizione generale

Glycogen phosphorylase catalyzes the rate-limiting step in glycogenolysis using glycogen and inorganic phosphate to produce glucose-1-phosphate (G1P). In mammals, glycogen phosphorylase is abundant in muscle, liver, and brain tissues. There are two forms of glycogen phosphorylase, namely glycogen phosphorylase a and b forms. Glycogen phosphorylase a is the highly active form whereas glycogen phosphorylase b has only limited activity. Glycogen phosphorylase is a clinically significant enzyme as its mutations are associated with different glycogen storage diseases in muscle and liver. In addition, this enzyme has been suggested as a biomarker for gastric cancer and its inhibition has been tested in treating type 2 diabetes.

Caratteristiche e vantaggi

Glycogen Phosphorylase Assay Kit:
  • Detects as low as 10 mU in a variety of sample types.

Compatibilità

The kit is suitable for the measurement of glycogen phosphorylase activity in cell culture and animal tissues (heart, liver, kidney, muscle, etc.) as well as the analysis of glycogen phosphorylase kinetics, inhibition or activation.

Principio

The Glycogen Phosphorylase Colorimetric Activity Assay Kit is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at A450 nm. The A450 nm signal is directly proportional to the glycogen phosphorylase activity. The kit is a rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity.

Pittogrammi

Health hazardCorrosion

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3


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