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H9394

Sigma-Aldrich

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Sinonimo/i:

HBSS

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.75

Sterilità

sterile-filtered

Forma fisica

liquid

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Componenti

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

Condizioni di spedizione

ambient

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Descrizione generale

Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.

Applicazioni

Hanks′ Balanced Salt solution has been used:
  • as a component of the culture medium for culturing of mouse cortical slices
  • as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)
  • for in situ perfusions of mice liver

Azioni biochim/fisiol

Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.

Supplemento

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

Trypsin Cell Dissociation Protocol

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

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