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DUO82040

Sigma-Aldrich

Duolink® In Situ Mounting Medium with DAPI

Sinonimo/i:

in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Nome Commerciale

Duolink®

tecniche

proximity ligation assay: suitable

Fluorescenza

λex 360 nm; λem 460 nm (Zeiss Filter set 49)

Compatibilità

suitable for fluorescence

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Categorie correlate

Applicazioni

Duolink® proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Use the Duolink® In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink®PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
Duolink® In Situ Mounting Medium with DAPI is ideal for nuclear staining and preserving signals generated with the Duolink® In Situ Detection Reagents for fluorescence microscopy. See datasheet for more details.
Note: Counterstaining with Cy®2 is not recommended.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list
Duolink® In Situ mounting medium with DAPI has been used to mount coverslips in proximity ligation assay (PLA) for nuclear staining.

Caratteristiche e vantaggi

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Note legali

Cy is a registered trademark of Cytiva
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

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N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Corrosion
GHS05

Avvertenze

Warning

Indicazioni di pericolo

H290,H412

Consigli di prudenza

P234 - P273 - P390 - P501

Classi di pericolo

Aquatic Chronic 3 - Met. Corr. 1

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Intraneuronal Amylin Deposition, Peroxidative Membrane Injury and Increased IL-1β Synthesis in Brains of Alzheimer?s Disease Patients with Type-2 Diabetes and in Diabetic HIP Rats
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Journal of Alzheimer'S Disease, 53(1), 259-272 (2016)
Detection of Receptor Heteromerization Using In Situ Proximity Ligation Assay
Gomes I, et al.
Current Protocols in Pharmacology / Editorial Board, S.J. Enna (editor-in-chief) ... [Et al.], 2-16 (2016)
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Nucleic Acids Research, 44(20), 9942-9955 (2016)
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Protein tyrosine kinase 6 (PTK6; breast tumour kinase) is overexpressed in up to 86% of the invasive breast cancers, and its association with the oncoprotein human epidermal growth factor receptor 2 (HER2) was shown in vitro by co-precipitation. Furthermore, expression
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Articoli

Protein-protein Interactions Detection with PLA

Proteins interact with various molecules, including other proteins, to fulfill complex cellular functions within biological systems.

Principio di funzionamento del saggio di ligazione di prossimità (PLA)

Scopri come funziona la tecnologia del saggio di ligazione di prossimità e come il kit per verificare l’interazione tra proteine permetta di confermare in situ la presenza di dimerizzazione di EGFR-HER2 indotta da EGF.

How Proximity Ligation Assays (PLA) Work

Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Protocolli

Duolink® PLA Multicolor Detection Protocol

Duolink® PLA Multicolor Detection Protocol

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

Duolink® PLA Fluorescence Protocol

This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.

Protocollo per PLA Duolink® in fluorescenza

Questo protocollo si usa per la rivelazione mediante immunofluorescenza di proteine presenti nelle cellule e nei tessuti.

Contenuto correlato

Protein & Nucleic Acid Interactions

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

Interazioni tra proteine e acidi nucleici

Reagenti e risorse per l’analisi delle interazioni tra proteine​e acidi nucleici per lo studio delle interazioni proteina-RNA, proteina-DNA e proteina-proteina e relative applicazioni.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.