A5153
Fosfatasi alcalina anti-IgG murina (molecola intera)−
affinity isolated antibody, buffered aqueous solution
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
Cambia visualizzazione
1 ML
CHF 309.00
About This Item
Prodotti consigliati
Origine biologica
goat
Coniugato
alkaline phosphatase conjugate
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Stato
buffered aqueous solution
tecniche
direct ELISA: 1:7,000-1:21,000
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
modifica post-traduzionali bersaglio
unmodified
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Categorie correlate
Descrizione generale
Affinity isolated antigen specific antibody is obtained from goat anti-mouse IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, that do not specifically bind to mouse IgG. Goat anti-Mouse IgG is conjugated to Sigma Alkaline Phosphatase, Type VII-S (Product No. P 5521) by protein crosslinking with 0.2% glutaraldehyde. Anti-Mouse IgG (Whole Molecule) is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP), versus mouse IgG and normal mouse serum, prior to conjugation. Prior to conjugation, the antibody is found to be reactive with mouse IgG1, IgG2a, IgG2b, and IgG by Ouchterlony Double Diffusion (ODD). Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation.
Specificità
Binds all mouse Igs.
mouse IgG1, IgG2a, IgG2b, and IgG
Immunogeno
Purified mouse IgG
Applicazioni
La proteina A tensioattiva è stata rinvenuta nel fluido broncoalveolare IgG di capra anti-murina coniugata con fosfatasi alcalina come secondaria a μg/mL in TBS/Tween contenente la concentrazione finale di NaCl 0,5 M.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Western blot analysis of nuclear or mitochondrial protein extracts were performed using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary antibody at a 1:5000 dilution in 5%BSA/TBS for 1 hour at room temperature. 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (Sigma) was used for the substrate.
Stato fisico
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Non trovi il prodotto giusto?
Prova il nostro Motore di ricerca dei prodotti.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Non trovi la versione di tuo interesse?
Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Siti Aisyah Abdul Ahmad et al.
Virology journal, 14(1), 229-229 (2017-11-23)
The rapid rise and spread in dengue cases, together with the unavailability of safe vaccines and effective antiviral drugs, warrant the need to discover and develop novel anti-dengue treatments. In this study the antiviral activity of geraniin, extracted from the
Xian Fu et al.
mBio, 8(5) (2017-09-07)
Methionine sulfoxide reductase A (MsrA) is an antioxidant enzyme found in all domains of life that catalyzes the reduction of methionine-
Lana J McMillan et al.
Applied and environmental microbiology, 82(2), 538-548 (2015-11-08)
Soluble inorganic pyrophosphatases (PPAs) that hydrolyze inorganic pyrophosphate (PPi) to orthophosphate (Pi) are commonly used to accelerate and detect biosynthetic reactions that generate PPi as a by-product. Current PPAs are inactivated by high salt concentrations and organic solvents, which limits
Tonia Zangari et al.
mBio, 8(2) (2017-03-16)
Epidemiological studies on Streptococcus pneumoniae show that rates of carriage are highest in early childhood and that the major benefit of the pneumococcal conjugate vaccine (PCV) is a reduction in the incidence of nasopharyngeal colonization through decreased transmission within a
An alternate form of Ku80 is required for DNA end-binding activity in mammalian mitochondria
Coffey, G.
Nucleic Acids Research, 20, 3793-3800 (2000)
Active Filters
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
