B5525

Bromophenol Blue sodium salt

• Sinonimo/i: 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
• Formula empirica (notazione di Hill): C₁₉H₉Br₄NaO₅S
• Numero CAS: 34725-61-6
• Peso molecolare: 691.94
• Beilstein: 6995701
• Numero CE: 252-170-2
• Numero MDL: MFCD00013793
• Codice UNSPSC: 12171500
• ID PubChem: 57653941
• NACRES: NA.52

Proprietà

• Stato: powder
• tecniche: electrophoresis: suitable
• Solubilità: water: soluble
• Compatibilità: suitable for nucleic acid electrophoresis
• Temperatura di conservazione: room temp
• Stringa SMILE: [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1; TWKDJMRHSLWRFI-UHFFFAOYSA-M

Descrizione

Descrizione generale

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Applicazioni

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis.[1] It has also been used in Drosphila fly food to aid visualization of gut for dissection.[2]

Ricostituzione

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)