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298R-1

Sigma-Aldrich

Myosin, Smooth Muscle (EP166) Rabbit Monoclonal Primary Antibody

Sinonimo/i:

Smooth Muscle Myosin, heavy chain (SMM-HC)

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About This Item

Codice UNSPSC:
12352203

Origine biologica

rabbit

Livello qualitativo

100
500

Coniugato

unconjugated

Forma dell’anticorpo

culture supernatant

Tipo di anticorpo

primary antibodies

Clone

EP166, monoclonal

Descrizione

For In Vitro Diagnostic Use in Select Regions

Forma fisica

buffered aqueous solution

Reattività contro le specie

human

Confezionamento

vial of 0.1 mL concentrate (298R-14)
vial of 0.1 mL concentrate Research Use Only (298R-14-RUO)
vial of 0.5 mL concentrate (298R-15)
vial of 1.0 mL concentrate (298R-16)
vial of 1.0 mL concentrate Research Use Only (298R-16-RUO)
vial of 1.0 mL pre-dilute Research Use Only (298R-17-RUO)
vial of 1.0 mL pre-dilute ready-to-use (298R-17)
vial of 7.0 mL pre-dilute ready-to-use (298R-18)
vial of 7.0 mL pre-dilute ready-to-use Research Use Only (298R-18-RUO)

Produttore/marchio commerciale

Cell Marque

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500 (concentrated)

Isotipo

IgG

Controllo

breast

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Visualizzazione

cytoplasmic

Informazioni sul gene

human ... MYH11(4629)

Descrizione generale

Smooth Muscle Myosin, heavy chain (SMM-HC) is a cytoplasmic structural protein that is a major component of the contractile apparatus of the smooth muscle cells. SMM-HC is also a myoepithelium-associated protein. Anti-SMM-HC is a monoclonal antibody to smooth muscle myosin, heavy chain that reacts with human visceral and vascular smooth muscle cells. The antibody also reacts with human myoepithelial cells. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.

Qualità


IVD

IVD

IVD

RUO

Linkage

Myosin, Smooth Muscle Positive Control Slides, Product No. 298S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Stato fisico

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.

Nota sulla preparazione

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Altre note

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Note legali

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Robert W Werling et al.
The American journal of surgical pathology, 27(1), 82-90 (2002-12-28)
Identification of myoepithelial cells using antibodies to cytoskeletal proteins, such as smooth muscle myosin heavy chain (SMM-HC) and calponin, can play an important role in distinguishing invasive carcinoma from its histologic mimics. However, antibodies to these proteins may also cross-react
D Lazard et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(3), 999-1003 (1993-02-01)
The expression of several differentiation markers in normal human mammary gland myoepithelium and in certain stromal fibroblasts ("myofibroblasts") associated with breast carcinomas was studied by immunofluorescence microscopy of frozen sections. Several antibodies to smooth muscle-specific proteins (smooth muscle alpha-actin, smooth
S N Agoff et al.
Applied immunohistochemistry & molecular morphology : AIMM, 9(2), 164-169 (2001-06-09)
Distinguishing low grade endometrial stromal sarcoma (ESS) from benign smooth muscle proliferations like cellular leiomyoma (CL) can be problematic; because of differing treatments and prognosis, this distinction is important. The authors tested the hypothesis that low grade ESS could be
Nikolay K Popnikolov et al.
American journal of clinical pathology, 120(2), 161-167 (2003-08-23)
We immunohistochemically compared benign myoepithelial tumors (adenomyoepitheliomas [AMEs]) and metaplastic matrix-producing (MMP-CA) and spindle cell (MSC-CA) carcinomas of the breast to identify helpful diagnostic markers. Normal myoepithelial cells (MECs) consistently expressed cytokeratin, alpha-smooth muscle actin (SMA), myosin, S-100, CD10, and

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