PRCYTMAG40K-PX24

MILLIPLEX^® Non-Human Primate Cytokine/Chemokine Panel II - 24-Plex Premixed Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.

• Codice UNSPSC: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Proprietà

• Livello qualitativo: 200
• Reattività contro le specie: nonhuman primates
• Produttore/marchio commerciale: Milliplex^®
• assay range: standard curve range: 1-1,500 pg/mL; (IL-17A); standard curve range: 10-10,000 pg/mL; (sCD137, IL-2, IP-10/CXCL10); standard curve range: 146-150,000 pg/mL; (IL-4); standard curve range: 15-15,000 pg/mL; (IL-21, IL-31); standard curve range: 20-20,000 pg/mL; (FGF-2, IL-33); standard curve range: 24-25,000 pg/mL; (IL-23); standard curve range: 29-30,000 pg/mL; (IL-1α, IL-28A); standard curve range: 293-300,000 pg/mL; (IL-17E/IL-25); standard curve range: 3-3,000 pg/mL; (Eotaxin/CCL11, IL-6); standard curve range: 4-4,000 pg/mL; (Granzyme B); standard curve range: 7-7,000 pg/mL; (MIP-3α/CCL20); standard curve range: 73-75,000 pg/mL; (IL-16); standard curve range: 78-80,000 pg/mL; (Granzyme A); standard curve range: 8-8,000 pg/mL; (sFasL); standard curve range: 98-100,000 pg/mL; (Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA)
• tecniche: multiplexing: suitable
• Compatibilità: configured for Premixed
• Metodo di rivelazione: fluorometric (Luminex xMAP)
• Condizioni di spedizione: wet ice

Descrizione

Descrizione generale

Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer.

The MILLIPLEX^® Non-Human Primate Premixed Cytokine Panel II is a 25 plex kit to be used for the simultaneous quantification of the following analytes in monkey serum, plasma, tissue/cell lysate and culture supernatant samples: sCD137, Eotaxin, sFASL, FGF-2, Fractalkine, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, MIP-3α, Perforin, RANTES and TNFβ.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificità

Cross Reactivty
This multiplexed assay panel has been tested with samples from multiple non-human primate species. Please see protocol for details.

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Applicazioni

• Analytes: sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, TNFβ/LTA
• Recommended Sample type: serum, plasma and cell culture samples
• Recommended Sample dilution: 1:2 diluted plasma or serum
• Assay Run Time: Overnight or one day. For best results, an overnight incubation is recommended
• Research Category: Inflammation & Immunology

Componenti

Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized

Sample Plate, 96 well format, 1 plate

2 Foil plate sealers

Assay Buffer, 2 bottles, 30 mL

Bead Diluent, 1 bottle, 3.5 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL

Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Premixed 24-plex Beads, 1 bottle, 3.5 mL

Stoccaggio e stabilità

Recommended storage for kit components is 2 - 8°C.

Altre note

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Note legali

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS06,GHS08,GHS05,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H302 + H332,H311,H317,H318,H373,H411
• Consigli di prudenza: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Classi di pericolo: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Organi bersaglio: Respiratory Tract
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects