PK1010

PhosphoDetect Anti-Syk (pTyr^525/526) Rabbit pAb

liquid, Calbiochem^®

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: liquid
• non contiene: preservative
• Reattività contro le specie: human, mouse
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pTyr525/pTyr526)
• Informazioni sul gene: human ... SYK(6850)

Descrizione

Descrizione generale

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr^525/526.

Recognizes the ~72 kDa Syk protein phosphorylated at Tyr^525/526 in human Syk or Tyr^519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.

This PhosphoDetect Anti-Syk (pTyr^525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr^525/526).

Immunogeno

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^525/526 phosphorylation sites of human Syk

Applicazioni

ELISA (1:1000)
Immunoblotting (1:1000)

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Ricostituzione

Do not aliquot.

Risultati analitici

Positive Control
293T cells transfected with wild-type Syk and CD8

Altre note

Coopman, P.J., et al. 2000. Nature406, 742.
Turner, M., et al. 2000. Immunol. Today21, 148.
Zang, J., et al. 2000. J. Biol. Chem.275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev.165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol.9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol.9, 309.

Tyr^525/526 represent the phosphorylation sites in human Syk, while Tyr^519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween^®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2