MABS1910

Anti-Signal peptide peptidase-like 3 Antibody, clone 7F9

clone 7F9, from mouse

• Sinonimo/i: Signal peptide peptidase-like 3, SPP-like 3, Intramembrane protease 2, IMP-2, Presenilin homologous protein 1, PSH1, Presenilin-like protein 4
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 7F9, monoclonal
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 25 μg
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_620584
• N° accesso UniProt: Q8TCT6
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SPPL3(121665)

Descrizione

Descrizione generale

Signal peptide peptidase-like 3 (UniProt: Q8TCT6; also known as SPP-like 3, Intramembrane protease 2, IMP-2, Presenilin homologous protein 1, PSH1, Presenilin-like protein 4, SPPL3) is encoded by the SPPL3 (also known as IMP2) gene (Gene ID: 121665) in human. SPPL3 is a multi-pass transmembrane protein that is shown to be widely expressed and is highly conserved in multicellular eukaryotes. Three isoforms of SPPL3 have been described that are produced by alternative splicing. It is a member of the intramembrane-cleaving GxGD proteases. It acts as an intramembrane protease that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries. It can also act as a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifying glycosidases and glycosyltransferases. It is also shown to catalyze the intramembrane cleavage of the envelope glycoprotein gp130 and/or the leader peptide gp18LP of the simian foamy virus independent of prior ectodomain shedding by furin or furin-like proprotein convertase-mediated cleavage proteolysis. The first transmembrane domain of SPPL3 may act as a type I signal anchor. SPPL3 also contains a PAL motif (aa 342-344) that is required for normal active site conformation.

Specificità

Clone 7F9 detects Signal peptide peptidase-like 3 in human cells. it targets an epitope within 15 amino acids from the C-terminal half. Immunogen sequence is conserved in all three isoforms.

Immunogeno

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human Signal peptide peptidase-like 3.

Applicazioni

Anti-Signal peptide peptidase-like 3, clone 7F9, Cat. No. MABS1910, is a mouse monoclonal antibody that detects Signal peptide peptidase-like 3 and has been tested for use in Immunoprecipitation and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected Signal peptide peptidase-like 3 in Western Blotting applications (Voss, M., et. al. (2012). J Biol Chem. 287(52):43401-9; Voss, M., et. al. (2014). EMBO J. 33(24):2890-905; Fleck, D., et. al. (2016). J Biol Chem. 291(1):318-33).

Immunoprecipitation Analysis: A representative lot detected Signal peptide peptidase-like 3 in Immunoprecipitation applications (Voss, M., et. al. (2012). J Biol Chem. 287(52):43401-9).

Qualità

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Signal peptide peptidase-like 3 in HEK293 cell lysate.

Descrizione del bersaglio

~42 kDa observed; 42.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash