MABE936

Anti-ASF-1 Antibody, clone AK-96

clone AK-96, from mouse

• Sinonimo/i: Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: AK-96, monoclonal
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_001071634
• N° accesso UniProt: Q07955
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SRSF1(6426)

Descrizione

Descrizione generale

Alternative-splicing factor 1 (ASF-1), also known as SRp30a or SF2, is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.

Immunogeno

Recombinant protein corresponding to human ASF-1.

Applicazioni

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-ASF-1 Antibody, clone AK-96 is validated for use in western blotting, IP & ICC for the detection of ASF-1.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of Raji and HepG2 cell lysate.
Immunocytochemistry Analysis: A representative lot detected ASF-1 in BJ fibroblast cells (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66).
Immunoprecipitation Analysis: A representative lot immunoprecipitated ASF-1 in BJ fibroblast cell lysate (Fregoso, O., et al. (2013). Mole. Cell. 50:56-66).

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ASF-1 in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~30 kDa observed

Linkage

Replaces: MABE512

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable