Yes, low-fluorescent PVDF membrane is required in Licor IR Imaging Systems. The Immobilon-FL PVDF Membrane is suitable for this purpose. Immobilon-FL Membrane is the first transfer membrane optimized for fluorescence applications, with extremely low background that improves the sensitivity of all fluorescence detection protocols. It is compatible with all commonly used fluorescent probes at all excitation and emission wavelengths, making it ideal for multiplexing and chemifluorescence applications.
IPFL07810
Immobilon®-FL PVDF Membrane
1 roll, 7 x 8.4cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
Sinonimo/i:
Western blotting membrane, blotting membrane, transfer membrane
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Informazioni su questo articolo
UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
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Permettici di aiutartiNome del prodotto
Membrana di trasferimento Immobilon®-FL in PVDF, 0.45 μm pore size, 7 X 8.4 cm sheets
material
PVDF membrane, plain filter, white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable, western blot: suitable
filter L × W
7 cm × 8.4 cm
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
detection method
chemiluminescent, colorimetric, fluorometric
shipped in
ambient
Quality Level
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Categorie correlate
1 of 4
Questo articolo | IPFL10100 | IPFL00010 | IPFL20200 |
|---|---|---|---|
| material PVDF membrane, white filter, plain filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter |
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| compatibility for use with Amido black, for use with Coomassie brilliant blue, for use with CPTS, for use with Ponceau-S red | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red | compatibility for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red |
| detection method chemiluminescent, fluorometric, colorimetric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® |
General description
La membrana di trasferimento Immobilon®-FL è una membrana microporosa di fluoruro di polivinilidene (PVDF) progettata per legare le proteine trasferite da una varietà di matrici di gel. La membrana di trasferimento Immobilon®-FL ha pori di dimensione nominale pari a 0,45 μm, ed è consigliata per il blotting di proteine con pesi molecolari superiori ai 20 kDa. La membrana è caratterizzata da un′autofluorescenza molto bassa in un ampio spettro di lunghezze d′onda di eccitazione/emissione. Tale proprietà la rende ideale per applicazioni di blotting che comportino una immunorivelazione basata sulla fluorescenza.
Codice filtro: IPFL
Codice filtro: IPFL
Application
Features and Benefits
Membrana di trasferimento Immobilon®-FL
- Ottimizzata per i metodi di immunorivelazione in fluorescenza
- La fluorescenza di fondo estremamente ridotta migliora la sensibilità con tutti i protocolli di immunorivelazione in fluorescenza
- Compatibile con tutte le comuni sonde fluorescenti, qualunque siano le lunghezze d'onda di eccitazione e di emissione
- Per rivelazioni standard in chemiluminescenza o rivelazione cromogena
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenuto correlato
Protein Blotting Survival Handbook: Tips and Tricks
Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.
There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.
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Could you confirm if the IPFL07810 Immobilon membrane is compatible with a specific imaging system? I need to verify if this product will work for my requirements.
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