AB110-I

Anti-PNMT Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: immunohistochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_001254480
• N° accesso UniProt: Q9UBM1
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... PEMT(10400)

Descrizione

Descrizione generale

PNMT, also known as Phosphatidylethanolamine N-methyltransferase, or PEMT, PEAMT, or PEMT2, and encoded by the gene PEMT/PEMPT/PNMT, catalyzes three sequential methylation reactions of phosphatidylethanolamine to produce phosphatidylcholine a major constituent of cell membranes and a primary pulmonary surfactant. PNMT/PEMT is localized to the endoplasmic reticulum membrane as well as the mitochondrial membranes. PNMT/PEMT is widely expressed, and has the highest activity in the mitochondria. The conversion of phosphatidylethanolamine to phosphatidylcholine by sequential methylation via PNMT/PEMT occurs in the liver. A different pathway for phosphatidylcholine production starting from choline also is present in cells but it does not involve PNMT/PEMT enzyme. Mutations in PNMT/PEMT protein are associated with nonalcoholic fatty liver disease (NAFLD).

Immunogeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human PNMT.

Applicazioni

Anti-PNMT Antibody detects level of PNMT & has been published & validated for use in PNMT.

Immunohistochemistry Analysis: A 1:500 dilution and a 1:2,000 dilution from a representative lot detected PNMT in human adrenal gland and testis tissues, respectively.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Qualità

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PNMT in 10 µg of human liver tissue lysate.

Descrizione del bersaglio

~17 kDa observed. As isoform of PNMT has been observed between 15-20 kDa (Cui, Z., et al. (1993). J Biol. Chem. 268(22):16655-16663.).

Linkage

Replaces: AB110

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable