420411

Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code.

• Sinonimo/i: Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested
• Formula empirica (notazione di Hill): C₁₈H₃₆N₄O₁₁ · xH₂SO₄
• Numero CAS: 25389-94-0
• Peso molecolare: 484.50 (free base basis)
• Codice UNSPSC: 12352200

Proprietà

• Livello qualitativo: 100
• Forma fisica: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Colore: white to off-white
• Solubilità: water: 10 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 10-30°C
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1; OOYGSFOGFJDDHP-KMCOLRRFSA-N

Descrizione

Descrizione generale

Recommended working concentration: 100 µg/ml.

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity.

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 70S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity. Useful for the selection of kanamycin resistant plasmids in E. coli. Tested and found to be negative for cytotoxicity against HeLa cells. Contains more than 98% kanamycin A.

Contains >98% kanamycin A. Potency: ≥750 µg/mg

Azioni biochim/fisiol

Primary Target
protein biosynthesis that acts on the 30S ribosome

Attenzione

Toxicity: Carcinogenic / Teratogenic (D)

Ricostituzione

Following reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Altre note

Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
Cardon, G.H., et al. 1993. Plant J.3, 773.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties1, 690.
Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother.6, 516.
Umezawa, S., et al. 1968. J. Antibiot.21, 367.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H360D
• Consigli di prudenza: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classi di pericolo: Repr. 1B
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 2