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HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series

HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

53970-U

Ascentis® Express HILIC HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

Étalon

Réf. du produit
Description
Tarif

S9378

Saccharose

≥99.5% (GC)

T0625

Taurine

≥99%

1286504

D-(−)-fructose

United States Pharmacopeia (USP) Reference Standard

G0350500

D-(+)-Glucose

European Pharmacopoeia (EP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)

flow rate

0.6 mL/min

pressure

812 psi (56 bar)

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

2 μL

sample

dilute1:9 in acetonitrile

Description

Remarque sur l'analyse

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UV and ELSD detectors in series, this chromatogram being from the ELSD.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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