Accéder au contenu
Merck

Polymeric nanoparticle-based delivery of microRNA-199a-3p inhibits proliferation and growth of osteosarcoma cells.

International journal of nanomedicine (2015-05-02)
Linlin Zhang, Arun K Lyer, Xiaoqian Yang, Eisuke Kobayashi, Yuqi Guo, Henry Mankin, Francis J Hornicek, Mansoor M Amiji, Zhenfeng Duan
RÉSUMÉ

Our prior screening of microRNAs (miRs) identified that miR-199a-3p expression is reduced in osteosarcoma cells, one of the most common types of bone tumor. miR-199a-3p exhibited functions of tumor cell growth inhibition, suggesting the potential application of miR-199a-3p as an anticancer agent. In the study reported here, we designed and developed a lipid-modified dextran-based polymeric nanoparticle platform for encapsulation of miRs, and determined the efficiency and efficacy of delivering miR-199a-3p into osteosarcoma cells. In addition, another potent miR, let-7a, which also displayed tumor suppressive ability, was selected as a candidate miR for evaluation. Fluorescence microscopy studies and real-time polymerase chain reaction results showed that dextran nanoparticles could deliver both miR-199a-3p and let-7a into osteosarcoma cell lines (KHOS and U-2OS) successfully. Western blotting analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays demonstrated that dextran nanoparticles loaded with miRs could efficiently downregulate the expression of target proteins and effectively inhibit the growth and proliferation of osteosarcoma cells. These results demonstrate that a lipid-modified dextran-based polymeric nanoparticle platform may be an effective nonviral carrier for potential miR-based anticancer therapeutics.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Diméthylsulfoxyde, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
Diméthylsulfoxyde, ACS reagent, ≥99.9%
Sigma-Aldrich
Diméthylsulfoxyde, for molecular biology
Sigma-Aldrich
Diméthylsulfoxyde, suitable for HPLC, ≥99.7%
Sigma-Aldrich
N,N-Diméthylformamide, ACS reagent, ≥99.8%
Sigma-Aldrich
Diméthylsulfoxyde, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
N,N-Diméthylformamide, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Diméthylsulfoxyde, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Pyridine, ACS reagent, ≥99.0%
Sigma-Aldrich
Lithium chloride, ACS reagent, ≥99%
Sigma-Aldrich
Diméthylsulfoxyde, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
Formamide, ReagentPlus®, ≥99.0% (GC)
Sigma-Aldrich
Sodium periodate, ACS reagent, ≥99.8%
Sigma-Aldrich
Cyanoborohydrure de sodium, reagent grade, 95%
Sigma-Aldrich
Diméthylsulfoxyde, puriss. p.a., ACS reagent, ≥99.9% (GC)
Sigma-Aldrich
Pyridine, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Potassium sulfate, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Sodium (meta)periodate, ≥99.0%
Sigma-Aldrich
2,2′-Azobis(2-methylpropionitrile), 98%
Sigma-Aldrich
N,N-Diméthylformamide, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.8% (GC)
Sigma-Aldrich
Pyridine, ReagentPlus®, ≥99%
Sigma-Aldrich
Formamide, BioReagent, ≥99.5% (GC), for molecular biology
Sigma-Aldrich
Chlorure d'acryloyle, ≥97%, contains ~400 ppm phenothiazine as stabilizer
Sigma-Aldrich
Lithium chloride, anhydrous, free-flowing, Redi-Dri, ReagentPlus®, 99%
Sigma-Aldrich
Lithium chloride, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99%
Sigma-Aldrich
N,N-Diméthylformamide, ReagentPlus®, ≥99%
Sigma-Aldrich
N,N-Diméthylformamide, for molecular biology, ≥99%
Sigma-Aldrich
Lithium chloride, for molecular biology, ≥99%
Sigma-Aldrich
Potassium sulfate, ACS reagent, ≥99.0%, powder
Sigma-Aldrich
Formamide, BioUltra, for molecular biology, ≥99.5% (T)