- Structure of AMP-PNP-bound BtuCD and mechanism of ATP-powered vitamin B12 transport by BtuCD-F.
Structure of AMP-PNP-bound BtuCD and mechanism of ATP-powered vitamin B12 transport by BtuCD-F.
Nature structural & molecular biology (2014-11-18)
Vladimir M Korkhov, Samantha A Mireku, Dmitry B Veprintsev, Kaspar P Locher
PMID25402482
RÉSUMÉ
The reaction mechanism of BtuCD-F-catalyzed vitamin B12 transport into Escherichia coli is currently unclear. Here we present the structure of the last missing state in the form of AMP-PNP-bound BtuCD, trapped by a disulfide cross-link. Our structural and biochemical data allow a consistent mechanism to be formulated, thus rationalizing the roles of substrate, ATP and substrate-binding protein.
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Vitamine B12, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%
Supelco
Vitamine B12, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Vitamine B12, European Pharmacopoeia (EP) Reference Standard