[Determination of fleroxacin by photochemical fluorescence of zinc-fleroxacin complex].

Based on the complex formed by Zn2+ which can strengthen the relative fluorescence intensity of fleroxacin evidently, a novel photochemical fluorescence method was developed. The effects of the acidity, the concentration ratio of Zn2+ to fleroxacin, and the time for illumination were studied. Under the optimum experiment conditions, the linear range of the determination was 5.00 x 10(-8) - 5.00 x 10(-6) mol x L(-1). The detection limit was 4.2 x 10(-8) mol x L(-1). The relative standard deviation of the determination of fleroxacin (5.0 x 10(-7) mol x L(-1)) was 1.7% (n = 20). The method was successfully applied to the determination of fleroxacin in specimens, and the recoveries were in the range of 95.0%-105%. The mechanism of this system is proposed.