Accéder au contenu
Merck

Influenza vaccine format mediates distinct cellular and antibody responses in human immune organoids.

Immunity (2023-07-22)
Jenna M Kastenschmidt, Suhas Sureshchandra, Aarti Jain, Jenny E Hernandez-Davies, Rafael de Assis, Zachary W Wagoner, Andrew M Sorn, Mahina Tabassum Mitul, Aviv I Benchorin, Elizabeth Levendosky, Gurpreet Ahuja, Qiu Zhong, Douglas Trask, Jacob Boeckmann, Rie Nakajima, Algimantas Jasinskas, Naresha Saligrama, D Huw Davies, Lisa E Wagar
RÉSUMÉ

Highly effective vaccines elicit specific, robust, and durable adaptive immune responses. To advance informed vaccine design, it is critical that we understand the cellular dynamics underlying responses to different antigen formats. Here, we sought to understand how antigen-specific B and T cells were activated and participated in adaptive immune responses within the mucosal site. Using a human tonsil organoid model, we tracked the differentiation and kinetics of the adaptive immune response to influenza vaccine and virus modalities. Each antigen format elicited distinct B and T cell responses, including differences in their magnitude, diversity, phenotype, function, and breadth. These differences culminated in substantial changes in the corresponding antibody response. A major source of antigen format-related variability was the ability to recruit naive vs. memory B and T cells to the response. These findings have important implications for vaccine design and the generation of protective immune responses in the upper respiratory tract.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Anti-Influenza A Antibody, nucleoprotein, clone A3, clone A3, Chemicon®, from mouse
Sigma-Aldrich
Anti-Influenza A Antibody, nucleoprotein, clone A1, FITC-conjugated, clone A1, Chemicon®, from mouse