Accéder au contenu
Merck

Orchiectomy but not adjuvant-induced arthritis induces structural modifications in rat aortas.

Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi (2022-08-10)
Agnaldo Bruno Chies, Maria Angélica Spadella, Carla Patrícia Carlos, Carla Brigagão Pacheco da Silva, Carlos Renato Tirapelli
RÉSUMÉ

This study aimed to verify whether Adjuvant-Induced Arthritis (AIA) and/or Orchiectomy (ORX) modify the expression of the Nox1, Nox2 and Nox4 isoforms, the endothelial function or the structure of rat aortas. Sixty-three Wistar rats were distributed into four groups: 1) Control; 2) ORX; 3) AIA; 4) Orchiectomy plus to Arthritis-induction (ORX/AIA). Thus, 21 days after the onset of AIA (by intradermal injection of Mycobacterium tuberculosis), the presence of Nox1, Nox2 and Nox4, the acetylcholine (ACh)-induced relaxation and the media layer thickness were assessed in the aorta taken from these animals. The Nox1, Nox2 and Nox4 were immunostained in intima, media and adventitia layers of aortas taken from all studied groups and AIA apparently increased this immunostaining. These modifications of Nox1, Nox2 or Nox4 expression, however, were not confirmed by Western blotting. In addition, neither AIA nor ORX changed the endothelial function, but ORX increased the media layer thickness in the studied aortas. The present study showed weak clues of increased expression of Nox1, Nox2 and Nox4 as a result of AIA, as well as of Nox1 reduction caused by ORX. In addition, the endothelial function was not modified in the aortas of these animals by both AIA and/or ORX. On the other hand, ORX increased significantly the aorta media layer thickness in the studied animals, which was apparently mitigated by AIA.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Anti-gp91-phox Antibody, Upstate®, from rabbit