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NMR waterLOGSY as An Assay in Drug Development Programmes for Detecting Protein-Ligand Interactions-NMR waterLOGSY.

Bio-protocol (2021-03-05)
Carole J R Bataille, Terence H Rabbitts, Timothy D W Claridge
RÉSUMÉ

In drug development programmes, multiple assays are needed for the determination of protein-compound interactions and evaluation of potential use in assays with protein-protein interactions. In this protocol we describe the waterLOGSY NMR method for confirming protein-ligand binding events.

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Sigma-Aldrich
Oxyde de deutérium, 99.9 atom % D
Sigma-Aldrich
Solution tampon phosphate, tablet
Sigma-Aldrich
Sulfoxyde de diméthyle-d6, Diméthylsulfoxyde-d6, "100%", 99.96 atom % D