Accéder au contenu
Merck

The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus.

Biochemical and biophysical research communications (2007-06-05)
Keiki Sugimoto, Hidenori Kage, Naomi Aki, Atsushi Sano, Hiroshi Kitagawa, Takahide Nagase, Yutaka Yatomi, Nobuya Ohishi, Daiya Takai
RÉSUMÉ

The field of epigenetics has made progress by the identification of the small RNA-mediated epigenetic modification. However, little is known about the key proteins. Here, we report that the human PIWI-like family is a candidate protein that is involved in the pathway responsible for chromatin remodeling. The PIWI-like family proteins, expressed as the Flag-fusion proteins, formed a bulky body and localized to the nuclear periphery. Transient transfection of PIWI-like 4 (PIWIL4), only member of the PIWI-like family that was ubiquitously expressed in human tissues, induced histone H3 lysine 9 methylation at the p16(Ink4a) (CDKN2A) locus. The elevated level of histone methylation resulted in the downregulation of the p16(Ink4a) gene. These results suggest PIWIL4 plays important roles in the chromatin-modifying pathway in human somatic cells.