P3125

Papaïne from papaya latex

buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein

• Synonyme(s) : Papaïnase
• Numéro CAS: 9001-73-4
• Numéro de classification (Commission des enzymes): 3.4.22.2 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131791
• Code UNSPSC : 12352204
• eCl@ss : 42030311
• Nomenclature NACRES : NA.54

Propriétés

• Forme: buffered aqueous suspension
• Qualité: 2× Crystallized
• Activité spécifique: ≥16 units/mg protein
• Poids mol.: 23.4 kDa
• Température de stockage: 2-8°C

Description

Description générale

Papain, a cysteine protease, consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group.

Application

Papain from papaya latex has been used to digest:

• Muller glial cells
• cartilage explants
• GAG and DNA content of the tissue constructs
• decellularized tissue samples.

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.

Sert à produire des fragments Fab d′anticorps. Également utilisée pour la dissociation cellulaire, car des études ont montré qu′elle s′avère plus efficace et moins détériorante avec certains tissus.

Actions biochimiques/physiologiques

Papain has broad specificity, in cleaving peptide bonds of basic amino acids, leucinehydrolyzes esters and amides. Papain digests many protein substrates extensively than pancreatic proteases. Papain is commonly used in cell isolation procedures since it is more efficient and less harmful compared to other proteases. Papain significantly increases the yield of viable smooth muscle cells without affecting the cell′s sensitivity to stimulants. It catalyzes several reactions, like hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Protéase à cystéine qui clive les liaisons peptidiques des acides aminés basiques, de la leucine ou de la glycine.
pH optimal situé entre 6,0 et 7,0.
Hydrolyse également les esters et les amides.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of N-α-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 °C.

Forme physique

Suspension in 0.05 M sodium acetate, pH 4.5, containing 0.01% thymol

Autres remarques

Pour en savoir plus sur la papaïne, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable