Accéder au contenu
Merck
Toutes les photos(2)

Principaux documents

MAK193

Sigma-Aldrich

Citrate Synthase Activity Assay Kit

Citrate Synthase Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonyme(s) :

Citrate Synthase Test Kit

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro CE :
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; cancer

Température de stockage

−20°C

Informations sur le gène

human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)

Description générale

Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.[1]

Application

Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.[2]

Adéquation

Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principe

The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.

Composants de kit seuls

Réf. du produit
Description

  • CS Assay Buffer

  • CS Substrate Mix

  • CS Developer

  • GSH Standard, Reduced

  • CS Positive Control

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3


Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 1

1 of 1

Md Habibur Rahman et al.
Microsystems & nanoengineering, 4, 39-39 (2019-05-07)
Defective mitochondria have been linked to several critical human diseases such as neurodegenerative disorders, cancers and cardiovascular disease. However, the detailed characterization of mitochondria has remained relatively unexplored, largely due to the lack of effective extraction methods that may sufficiently
Xi Lin et al.
International journal of molecular sciences, 21(3) (2020-01-26)
Maintaining an active fatty acid metabolism is important for renal growth, development, and health. We evaluated the effects of anaplerotic and ketogenic energy sources on fatty acid oxidation during stimulation with clofibrate, a pharmacologic peroxisome proliferator-activated receptor α (PPARα) agonist.
Tony Chao et al.
American journal of physiology. Cell physiology, 317(6), C1229-C1238 (2019-09-19)
Severe burn injury results in systemic disruption of metabolic regulations and impaired cardiac function. Restoration of hemodynamic homeostasis utilizing intravenous (IV) fluids is critical for acute care of the burn victim. However, the effects of burns and resuscitation on cardiomyocyte
P Dongil et al.
Scientific reports, 8(1), 13810-13810 (2018-09-16)
Metabolic dysfunction in the liver is the cause of numerous pathologies, which are associated with an altered redox state. PASK (PAS Domain Kinase) is a nutrient and bioenergetic sensor. We contend that PASK could act as an oxidative stress sensor
Regulation of Expression of Citrate Synthase by the Retinoic Acid Receptor-Related Orphan Receptor α (RORα)
Crumbley C, et al.,
PLoS ONE, 7, e33804 -e33804 (2012)

Questions

  1. Is the sensitivity information for the CS0720 (Citrate Synthase Assay Kit) available?

    1 answer
    1. The lower concentration tested for the product CS0720 (Citrate Synthase Assay Kit) is 1u/µgP

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 0
    1 out of 5 stars.

    This actually measures total deacetylation activity, not specific to CS

    The mechanism for the assay kit is the deacetylation of Acetyl-CoA to CoA. There are multiple enzymes can do this, not just CS. This assay kit doesn't have a good control for the background non-CS-related deacetylation step, so it is not the right product to measure CS activity. CS0720 is the CS specific assay kit to buy.

    Helpful?

    1. Response from MilliporeSigma:

      MAK193 has been discontinued, CS0720 is its recommended replacement. CS0720 includes a positive control (which MAK193 lacked). CS0720 provides the Acetyl Coenzyme A and the Oxaloacetic acid needed for Citrate Synthase to produce Citrate.

      We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique