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GE28-9466-03

HiTrap® Albumin IgG Depletion

Cytiva 28-9466-03, pack of 2 × 1 mL

Synonyme(s) :

Albumin depletion column, Albumin removal column

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About This Item

Code UNSPSC :
41105323
Nomenclature NACRES :
NA.56

ligand

recombinant protein G fragment and recombinant protein binding HSA

Caractéristiques

wetted part: no

Conditionnement

pack of 2 × 1 mL

Fabricant/nom de marque

Cytiva 28-9466-03

Conditions de stockage

(20% Ethanol)

Taille du lit

7 mm × 25 mm

Volume du lit

1 mL

D.I. de la colonne

7 mm

Taille moy. des particules

34 μm

cleaning

2-9

Plage de travail

3-9

Température de stockage

2-8°C

Description générale

HiTrap® Albumin & IgG Depletion is prepacked columns for the depletion of albumin and IgG from human serum and plasma. The columns are prepacked with high performance SepharoseTM based media with an affinity for human serum albumin (HSA) and IgG.

Application

HiTrap® Albumin & IgG Depletion and Albumin & IgG Depletion SpinTrap are prepacked columns for the depletion of albumin and IgG from plasma and serum samples. Albumin and IgG are the most abundant plasma Proteins, and they tend to obscure the signals of less abundant Proteins. Depletion of the two Proteins removes more than 60% of the total Protein content in human plasma, allowing a higher load of less abundant Proteins to be included in the analysis and detection of more Proteins. These columns are members of the Trap platform and address the need for flexible, small-scale preparation of Protein samples prior to downstream analyses such as 1-D or 2-D gel electrophoresis and mass spectrometry.

Caractéristiques et avantages

  • HiTrap® Albumin & IgG Depletion is a prepacked column for the depletion of albumin and IgG from human serum or plasma.
  • HiTrap® Albumin & IgG Depletion column provides rapid and easy processing in a convenient format for handling larger sample volumes (~150 μL) using a liquid chromatography system or manually using a syringe.
  • Columns are prepacked with high performance Sepharose based media with an affinity for human serum albumin (HSA) and IgG.
  • High depletion capacity, removes > 95% HSA and > 90% IgG, with high reproducibility.
  • Simple and fast procedure.
  • Removal of albumin and IgG allows a higher load of less abundant Proteins to be included in the analysis and detection of more Proteins.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


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Certificats d'analyse (COA)

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Protocoles

This page shows how to purify or remove albumin and IgG with Albumin & IgG Depletion Sepharose High Performance from Cytiva.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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