11721933001
Roche
DNA Molecular Weight Marker XIV (100 bp ladder)
solution, pkg of 50 μg (in 200 μl)
Synonyme(s) :
DNA marker
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41105335
Produits recommandés
Forme
solution
Niveau de qualité
Conditionnement
pkg of 50 μg (in 200 μl)
Fabricant/nom de marque
Roche
Concentration
250 μg/mL
Couleur
colorless
Solubilité
water: miscible
Température de stockage
−20°C
Description générale
Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 100 to 1500 bp and an additional band of 2642 bp
Size Range: 100 to 1500 bp and an additional band of 2642 bp
Application
DNA Molecular Weight Marker XIV (100 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest or RT-PCR, following separation on agarose gels. The DNA molecular weight marker can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder) for precise size determination.
Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
DNA Molecular Weight Marker XIV has been used in gel electrophoresis.
Séquence
The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, and an additional band of 2642 bp. The 500-bp and 1000-bp banding pattern is two to three times brighter.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.To make them easy to identify, the 500 and 1,000 bp bands are two to three times as bright as the other bands in the ladder.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.To make them easy to identify, the 500 and 1,000 bp bands are two to three times as bright as the other bands in the ladder.
Définition de l'unité
50 μg = 1A260 unit
Forme physique
Ready-to-use solution, 250 μg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Mirco Fanelli et al.
Nature protocols, 6(12), 1905-1919 (2011-11-16)
Formalin-fixed, paraffin-embedded (FFPE) samples represent the gold standard for storage of pathology samples. Here we describe pathology tissue chromatin immunoprecipitation (PAT-ChIP), a technique for extraction and high-throughput analysis, by techniques such as ChIP-seq, of chromatin derived from FFPE samples. Technically
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique