MABT390

Anti-Syntaxin-2 Antibody, clone 2F2.1

clone 2F2.1, from mouse

• Synonyme(s) : Syntaxin-2, Epimorphin
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2F2.1, monoclonal
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_001971
• Numéro d'accès UniProt: P32856
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... STX2(2054)

Description

Description générale

The protein named Syntaxin-2 or Epimorphin is a multifunctional membrane protein with dual topology. Its C terminus is membrane anchored. When the N terminus is on the cytosolic face it acts as a t-SNARE involved in intracellular vesicle docking and is called Syntaxin-2. When flipped inside out the N terminus hangs out on the extracellular surface (by some nonclassical secretion pathway) it acts as a versatile morphogen and is called Epimorphin. This membrane protein enjoys the double choice of another form of topological alternatives of being targeted to either apical or basolateral surface of an epithelial cell in a regulated way depending on various contexts. When expressed by mesenchymal cells it can instruct epithelial morphogenesis at epithelial mesenchymal interfaces, and Syntaxin-2 is protein that is essential for epithelial cell and tissue morphogenesis and synaptic function in nervous system communication. Syntaxin-2 interacts with synaptotagmin-6 (SYT6) and synaptotagmin-8 (SYT8) in calcium dependent exocytosis of synaptic vesicles and may mediate calcium regulation of exocytosis in the acrosomal reaction in sperm cells as well.

Immunogène

GST-tagged recombinant protein corresponding to human Syntaxin-2.

Application

Anti-Syntaxin-2 Antibody, clone 2F2.1 is a highly specific mouse monoclonal antibody, that targets Syntaxin 2 & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Syntaxin-2 in SH-SY5Y cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Qualité

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Syntaxin-2 in 200 µg of SH-SY5Y cell lysate.

Description de la cible

~ 33 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable