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AQ502B

Sigma-Aldrich

Goat Anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed

0.5 mg/mL, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

Conjugué

biotin conjugate

Forme d'anticorps

purified antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Espèces réactives

mouse

Fabricant/nom de marque

Chemicon®

Concentration

0.5 mg/mL

Technique(s)

ELISA: suitable
immunofluorescence: suitable

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Description générale

IgG is the most abundant immunoglobulin and is comprised of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Spécificité

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Immunogène

Epitope: Mouse IgM, IgG, IgA
Prepared from pepsin digest of mouse Ig (H+L).

Application

Goat anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, biotin conjugate, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA & IF.
Immunofluorescence: ≤ 1 μg/106 cells.
ELISA: 1:5,000-1:20,000.

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies

Forme physique

Gel filtration chromatography
Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 1.0 mL PBS/NaN3.

Stockage et stabilité

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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