07-2270

Anti-WNK4 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Serine/threonine-protein kinase WNK4, Protein kinase lysine-deficient 4, Protein kinase with no lysine 4
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, mouse
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_115763
• Numéro d'accès UniProt: Q96J92
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... WNK4(65266)

Description

Description générale

WNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis.

Spécificité

This antibody recognizes WNK4 at the C-terminus.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human WNK4 at the C-terminus.

Application

Immunohistochemistry Analysis: 1:1,000 dilution from a previous lot detected WNK4 in normal human kidney tissue.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

This WNK4 antibody is validated for use in WB & IHC for the detection of the WNK4 protein.

Qualité

Evaluated by Western Blot in mouse kidney cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected WNK4 on 10 µg of mouse kidney cell lysate.

Description de la cible

~160 kDa observed. This protein has 3 isoforms produced by alternative splicing. Hengl, T., et al. (2010). PNAS. 107(13):6052–6057.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Mouse kidney cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable