L021000

DCA Deblock

with Dichloromethane

• Code UNSPSC : 41116004
• Nomenclature NACRES : NA.21

Propriétés

• Type: for DNA synthesis; for DNA synthesis
• Gamme de produits: Proligo Reagents
• Forme: liquid
• Conditions de stockage: dry at room temperature
• Concentration: 42.2-51.6 g/L (DCA content, titration)
• Impuretés: ≤200 ppm water content (Karl Fischer)
• Couleur: clear

Description

Description générale

Deblock solution contains dichloroacetic acid (DCA) in dichloromethane that assists in the removal of the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, before the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Notes préparatoires

Dicloromethane / Dichloroacetic acid 97/3 (v/v)

Autres remarques

Deblock solution containing dichloroacetic acid (DCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H318,H336,H351,H360FD,H362
• Conseils de prudence: P202 - P263 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Carc. 2 - Eye Dam. 1 - Lact. - Repr. 1B - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Central nervous system
• Code de la classe de stockage: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable