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Merck

Protein Research

Extraction, isolation, purification, separation, detection...

We know your time is better spent analyzing data than performing arduous protocols before you can even measure your proteins of interest. We can help with the most efficient approaches to cloning and expression with a wide variety of cells, reagents, and vectors.  

Our membrane engineers have developed devices expressly for desalting, concentrating, and buffer exchange that saves your proteins and save you time and protocol steps. A comprehensive offering of protein preparation products provides gentle extraction, high-yield isolation and purification, and application-verified detection tools for techniques like electrophoresis and Western blot, ELISA, multiplex, and mass spectrometry.

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From videos to detailed protocols, technical articles, and application notes, you’ll find a wealth of information to keep your research moving forward.

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Related Applications
Cloning & Expression
Cloning, protein expression vectors and techniques for optimal gene cloning and protein expression systems.
PCR Applications
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
Nucleic Acid Gel Electrophoresis
Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge for cloning, PCR, mass spectrometry, next generation sequencing (NGS), and Northern and Southern blotting applications.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
DNA & RNA Purification
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Protein Expression
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
Lysis & Protein Extraction
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
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