HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)
Materials
standard
Product No.
Description
Pricing
Cytochrome c from bovine heart
≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell cultureCytochrome c from equine heart
BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell cultureCONDITIONS
column
Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)
mobile phase
[A] 20 mM Bis-Tris HCl, pH 7.0; [B] 20 mM Bis-Tris HCl, 0.5M NaCl, pH 7.0; 24 to 69% B in 20 min. (linear)
flow rate
0.5 mL/min
column temp.
25 °C
detector
UV, 280 nm
injection
10 μg each variant
Description
Analysis Note
This Figure shows the separation of three cytochrome c variants on Discovery BIO PolyMA-SCX. Note the difference in elution order compared to the reversed-phase separation on Discovery BIO Wide Pore C18 shown in product G001581.
Legal Information
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