Spherically-clustered porous Au-Ag alloy nanoparticle prepared by partial inhibition of galvanic replacement and its application for efficient multimodal therapy.

Spherically-clustered porous Au-Ag alloy nanoparticle prepared by partial inhibition of galvanic replacement and its application for efficient multimodal therapy.

ACS nano (2015-01-07)

Hongje Jang, Dal-Hee Min

ABSTRACT

The polyvinylpyrrolidone (PVP)-coated spherically clustered porous gold-silver alloy nanoparticle (PVP-SPAN) was prepared by low temperature mediated, partially inhibited galvanic replacement reaction followed by silver etching process. The prepared porous nanostructures exhibited excellent photothermal conversion efficiency under irradiation of near-infrared light (NIR) and allowed a high payload of both doxorubicin (Dox) and thiolated dye-labeled oligonucleotide, DNAzyme (FDz). Especially, PVP-SPAN provided 10 times higher loading capacity for oligonucleotide than conventional hollow nanoshells due to increased pore diameter and surface-to-volume ratio. We demonstrated highly efficient chemo-thermo-gene multitherapy based on codelivery of Dox and FDz with NIR-mediated photothermal therapeutic effect using a model system of hepatitis C virus infected human liver cells (Huh7 human hepatocarcinoma cell line containing hepatitis C virus NS3 gene replicon) compared to conventional hollow nanoshells.

MATERIALS

Product Number

Brand

Product Description

S4132

Sigma-Aldrich

Sodium carbonate monohydrate, ReagentPlus^®, ≥99.5%

H3410

Sigma-Aldrich

Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H₂O, meets USP testing specifications

95299

Sigma-Aldrich

Hydrogen peroxide solution, purum p.a., ≥35% (RT)

13568

Sigma-Aldrich

Sodium carbonate monohydrate, puriss., meets analytical specification of Ph. Eur., BP, NF, FCC, E500, 99.5-100.5% (ex dried subst.)

323381

Sigma-Aldrich

Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H₂O

349887

Sigma-Aldrich

Hydrogen peroxide solution, contains inhibitor, 35 wt. % in H₂O

230952

Sigma-Aldrich

Sodium carbonate monohydrate, ACS reagent, ≥99.5%

216763

Sigma-Aldrich

Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H₂O, ACS reagent

516813

Sigma-Aldrich

Hydrogen peroxide solution, 50 wt. % in H₂O, stabilized

31642

Sigma-Aldrich

Hydrogen Peroxide Solution, 30% (w/w), puriss. p.a., reag. ISO, reag. Ph. Eur.

D2975000

Doxorubicin hydrochloride, European Pharmacopoeia (EP) Reference Standard

16911

Supelco

Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis

11627

Sigma-Aldrich

Diethyl azodicarboxylate solution, purum, ~40% in toluene (H-NMR)

18304

Sigma-Aldrich

Hydrogen peroxide solution, 34.5-36.5%

95321

Supelco

Hydrogen peroxide solution, ≥30%, for trace analysis

44583

Sigma-Aldrich

Doxorubicin hydrochloride, suitable for fluorescence, 98.0-102.0% (HPLC)

95294

Sigma-Aldrich

Hydrogen peroxide solution, tested according to Ph. Eur.

49629

Sigma-Aldrich

Glutaraldehyde solution, technical, ~50% in H₂O (5.6 M)

340855

Sigma-Aldrich

Glutaraldehyde solution, 50 wt. % in H₂O

G6403

Sigma-Aldrich

Glutaraldehyde solution, 50% in H₂O, suitable for photographic applications

G6257

Sigma-Aldrich

Glutaraldehyde solution, Grade II, 25% in H₂O

G7526

Sigma-Aldrich

Glutaraldehyde solution, Grade I, 8% in H₂O, specially purified for use as an electron microscopy fixative or other sophisticated use

G5882

Sigma-Aldrich

Glutaraldehyde solution, Grade I, 25% in H₂O, specially purified for use as an electron microscopy fixative

D1515

Sigma-Aldrich

Doxorubicin hydrochloride, 98.0-102.0% (HPLC)

M2128

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide, 98%

M5655

Sigma-Aldrich

Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

H1009

Sigma-Aldrich

Hydrogen peroxide solution, 30 % (w/w) in H₂O, contains stabilizer

G7651

Sigma-Aldrich

Glutaraldehyde solution, Grade I, 50% in H₂O, specially purified for use as an electron microscopy fixative or other sophisticated use

G7776

Sigma-Aldrich

Glutaraldehyde solution, Grade I, 70% in H₂O, specially purified for use as an electron microscopy fixative or other sophisticated use

88597

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology