Silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent Pseudomonas putida in laboratory medium and artificial wastewater.

Silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent Pseudomonas putida in laboratory medium and artificial wastewater.

Environmental pollution (Barking, Essex : 1987) (2014-09-28)

Florian Mallevre, Teresa F Fernandes, Thomas J Aspray

ABSTRACT

Bacteria based ecotoxicology assessment of manufactured nanoparticles is largely restricted to Escherichia coli bioreporters in laboratory media. Here, toxicity effects of model OECD nanoparticles (Ag NM-300K, ZnO NM-110 and TiO2 NM-104) were assessed using the switch-off luminescent Pseudomonas putida BS566::luxCDABE bioreporter in Luria Bertani (LB) medium and artificial wastewater (AW). IC50 values ∼4 mg L(-1), 100 mg L(-1) and >200 mg L(-1) at 1 h were observed in LB for Ag NM-300K, ZnO NM-110 and TiO2 NM-104, respectively. Similar results were obtained in AW for Ag NM-300K (IC50∼5 mg L(-1)) and TiO2 NM-104 (IC50>200 mg L(-1)) whereas ZnO NM-110 was significantly higher (IC50>200 mg L(-1)). Lower ZnO NM-110 toxicity in AW compared to LB was associated with differences in agglomeration status and dissolution rate. This work demonstrates the importance of nanoecotoxicological studies in environmentally relevant matrices.

MATERIALS

Product Number

Brand

Product Description

700347

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanoparticles, <150 nm particle size (volume distribution, DLS), dispersion, 40 wt. % in H₂O, 99.5% trace metals basis

739448

Sigma-Aldrich

Silver nanowires, diam. × L 100 nm × 6 μm, 0.5% (isopropyl alcohol suspension)

637262

Sigma-Aldrich

Titanium(IV) oxide, rutile, nanopowder, <100 nm particle size, 99.5% trace metals basis

634662

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanopowder, <100 nm particle size (BET), 99.5% trace metals basis

IRMM531B

Titanium, IRMM^®, certified reference material, 0.5 mm foil

635057

Sigma-Aldrich

Titanium(IV) oxide, rutile, <001>, (single crystal substrate), ≥99.9% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm

739421

Sigma-Aldrich

Silver nanowires, diam. × L 50 nm × 6 μm, 0.5% (isopropyl alcohol suspension)

85131

Sigma-Aldrich

Silver, colloidal, 65-75% Ag basis

348783

Sigma-Aldrich

Silver, wire, diam. 0.1 mm, 99.9% trace metals basis

348791

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis

266035

Sigma-Aldrich

Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

IRMM531C

Titanium, IRMM^®, certified reference material, 0.5 mm wire

224227

Sigma-Aldrich

Titanium(IV) oxide, rutile, powder, <5 μm, ≥99.9% trace metals basis

204730

Sigma-Aldrich

Titanium(IV) oxide, rutile, 99.995% trace metals basis

204757

Sigma-Aldrich

Titanium(IV) oxide, rutile, ≥99.98% trace metals basis

369489

Sigma-Aldrich

Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis

484059

Sigma-Aldrich

Silver, nanopowder, <150 nm particle size, 99% trace metals basis

327050

Sigma-Aldrich

Silver, shot, 1-3 mm, ≥99.99% trace metals basis

267902

Sigma-Aldrich

Titanium, wire, diam. 0.81 mm, 99.7% trace metals basis

265608

Sigma-Aldrich

Silver, wire, diam. 1.0 mm, 99.9% trace metals basis

265586

Sigma-Aldrich

Silver, wire, diam. 0.5 mm, ≥99.99% trace metals basis

265594

Sigma-Aldrich

Silver, wire, diam. 1.0 mm, ≥99.99% trace metals basis

326976

Sigma-Aldrich

Silver, foil, thickness 2.0 mm, 99.9% trace metals basis

460400

Sigma-Aldrich

Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis

265500

Sigma-Aldrich

Silver, powder, <250 μm, 99.99% trace metals basis

295744

Sigma-Aldrich

Silver, wool, diam. 0.05 mm, ≥99.9% trace metals basis

305812

Sigma-Aldrich

Titanium, 5-10 mm, ≥99.99% trace metals basis (purity exclusive of Na and K content)

265551

Sigma-Aldrich

Silver, wire, diam. 0.127 mm, 99.99% trace metals basis

265527

Sigma-Aldrich

Silver, foil, thickness 0.1 mm, 99.9% trace metals basis

369438

Sigma-Aldrich

Silver, foil, thickness 1.0 mm, 99.99% trace metals basis