Skip to Content

Markers of dopamine metabolism in Parkinson's disease. The Parkinson Study Group.

Markers of dopamine metabolism in Parkinson's disease. The Parkinson Study Group.

Neurology (1992-11-01)

P A LeWitt, M P Galloway, W Matson, P Milbury, M McDermott, D K Srivastava, D Oakes

ABSTRACT

We used two analytic methods (a multichannel coulometric electrode array with high-performance liquid chromatography, and gas chromatography-mass spectrophotometry) to measure CSF dopamine (DA) and its metabolites in mildly affected, unmedicated subjects with Parkinson's disease (PD). The mean (+/- SD) concentration of homovanillic acid (HVA), the most abundant product of DA turnover, was 164.57 +/- 95.05 nM. As sequential aliquots of CSF were collected from the first to 23rd ml, CSF HVA concentration almost doubled. After HVA, 3-O-methyldopa (3-O-MD) was the next most abundant compound. The summed concentrations of 3-O-MD, 3,4-dihydroxyphenylacetic acid, 3-methoxytyramine, DA, DA-3-sulfate, homovanillol, and levodopa (LD) amounted to 12.6% of HVA. Concentrations of the DA metabolites did not correlate to a variety of indices of PD severity. The presence of LD and 3-O-MD may be indicators of DA synthesis and possibly could reflect compensatory processes among surviving dopaminergic neurons of the PD brain.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Calcium, dendritic pieces, purified by distillation, 99.9% trace metals basis

Sigma-Aldrich

Aluminum, foil, thickness 0.25 mm, 99.999% trace metals basis

Sigma-Aldrich

Copper, powder (dendritic), <45 μm, 99.7% trace metals basis

Sigma-Aldrich

Copper, foil, thickness 0.25 mm, 99.98% trace metals basis

Sigma-Aldrich

Zinc, mossy, ≥99%

Sigma-Aldrich

Titanium, powder, −100 mesh, 99.7% trace metals basis

Sigma-Aldrich

Titanium, sponge, 1-20 mm, 99.5% trace metals basis

Sigma-Aldrich

Silicon, powder, −325 mesh, 99% trace metals basis

Sigma-Aldrich

Aluminum, foil, thickness 1.0 mm, 99.999% trace metals basis

Sigma-Aldrich

Nickel, wire, diam. 0.25 mm, ≥99.9%

Sigma-Aldrich

Titanium, evaporation slug, diam. × L 6.3 mm × 6.3 mm, ≥99.99% trace metals basis

Sigma-Aldrich

Taurine, ≥98%, FG

Sigma-Aldrich

Bismuth, pieces, 1-12 mm, 99.999% trace metals basis

Sigma-Aldrich

Aluminum, foil, thickness 0.13 mm, ≥99.99% trace metals basis

Supelco

Taurine, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

Tungsten, wire, diam. 0.5 mm, ≥99.9% trace metals basis

Sigma-Aldrich

Tin, powder, <45 μm particle size, 99.8% trace metals basis

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, ≥99.99% trace metals basis

Sigma-Aldrich

Bismuth, powder, −100 mesh, 99% trace metals basis

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 3202, slurry, in H₂O, active catalyst

Sigma-Aldrich

Zinc, wire, diam. 1.0 mm, 99.995% trace metals basis

Sigma-Aldrich

Bismuth, shot, 4-30 mesh, 99.9% trace metals basis

Sigma-Aldrich

Nickel, powder, <150 μm, 99.99% trace metals basis

Copper (O), BCR^®, certified reference material, rod

Sigma-Aldrich

Raney^®-Nickel, W.R. Grace and Co. Raney^® 2800, slurry, in H₂O, active catalyst

Sigma-Aldrich

Bismuth, powder, −100 mesh, ≥99.99% trace metals basis

Sigma-Aldrich

Zinc, granular, 30-100 mesh, 99%

Copper, IRMM^®, certified reference material, 0.5 mm wire

SAFC

Taurine

Sigma-Aldrich

Bismuth, granular, ≥99.99% trace metals basis