- A colorimetric microassay for the detection of agents that interact with DNA.
A colorimetric microassay for the detection of agents that interact with DNA.
Journal of natural products (1992-11-01)
N S Burres, A Frigo, R R Rasmussen, J B McAlpine
PMID1479377
ABSTRACT
A simple microtiter assay for the detection of compounds that bind DNA is described. Agents that displace methyl green from DNA are detected spectrophotometrically by a decrease in absorbance at 630 nm. The feasibility of using the assay for detecting DNA-active compounds in fermentation extracts was assessed, and the activities of reference compounds in the methyl green assay and an ethidium bromide displacement method were compared.