Poly (dextrogyr-levogyr) lactide acid-triiodothyronine scaffold for peripheral nerve regeneration.

Poly (dextrogyr-levogyr) lactide acid-triiodothyronine scaffold for peripheral nerve regeneration.

Neuro endocrinology letters (2013-03-26)

Min Wang, Chao Liu, Yong Hao, Yuan Zhang, Xia Zhang

ABSTRACT

To evaluate the poly(dextrogyr-levogyr) lactide acid-triiodothyronine (PDLLA-T3) seeded with Schwann cells conduit for repairing sciatic nerve defect. The rats were divided into three groups: autologous nerve transplantation (Group A), PDLLA-T3 + Schwann cells (Group B) and PDLLA + Schwann cells (Group C). Myelin sheath thickness was significantly greater in Group A compared with Group B and Group C. The regenerated nerves had nearly normal structure in Group A, and in Groups B and C nerve tissues filled in the anastomotic site and angiogenesis was noted. The mean number of myelinated nerve fibers and neurons in Group B was greater than in Group C. PDLLA-T3 is superior to PDLLA alone for repairing nerve defects.

MATERIALS

Product Number

Brand

Product Description

T2877

Sigma-Aldrich

3,3′,5-Triiodo-L-thyronine, ≥95% (HPLC), powder

T-074

Supelco

3,3′,5-Triiodo-L-thyronine (T₃) solution, 100 μg/mL in methanol with 0.1N NH₃, ampule of 1 mL, certified reference material, Cerilliant^®

38534

Sigma-Aldrich

Polylactic acid, M_w ~60,000