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  • An efficient and economical MTT assay for determining the antioxidant activity of plant natural product extracts and pure compounds.

An efficient and economical MTT assay for determining the antioxidant activity of plant natural product extracts and pure compounds.

Journal of natural products (2010-06-23)
Yunbao Liu, Muraleedharan G Nair
ABSTRACT

Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected natural product extracts and purified compounds. This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput.

MATERIALS
Product Number
Brand
Product Description

Quercitrin, primary reference standard
SAFC
Cholesterol, Plant-Derived, SyntheChol®
Sigma-Aldrich
trans-Ferulic acid, ≥99%
Sigma-Aldrich
Chrysin, ≥96.5%
Sigma-Aldrich
Undecanoic acid, 98%
Sigma-Aldrich
trans-Ferulic acid, 99%
Supelco
Ursolic acid, analytical standard
Supelco
Stearic acid, analytical standard
Sigma-Aldrich
2,6-Di-tert-butyl-4-methylphenol, tested according to Ph. Eur.
Supelco
Undecanoic acid, analytical standard
Sigma-Aldrich
Methyl linolenate, technical, 70-80% (GC)
Sigma-Aldrich
Undecanoic acid, ≥99%, FG
Sigma-Aldrich
3-(4-Hydroxyphenyl)propionic acid, 98%
Sigma-Aldrich
4-Hydroxybenzoic acid, ReagentPlus®, 99%
Sigma-Aldrich
4-Hydroxybenzoic acid, ReagentPlus®, ≥99%
Supelco
Dodecanoic acid, analytical standard
Supelco
trans-Ferulic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)
Sigma-Aldrich
D-Camphor, ≥97%, FG
Sigma-Aldrich
Stearic acid, reagent grade, 95%
Sigma-Aldrich
Quercetin hydrate, ≥95%
Sigma-Aldrich
Dodecanoic acid, 98%
Supelco
Nervonic acid, analytical standard
Sigma-Aldrich
2,6-Di-tert-butyl-4-methylphenol, purum, ≥99.0% (GC)
Sigma-Aldrich
Stearic acid, ≥95%, FCC, FG
Supelco
Caffeic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)
Sigma-Aldrich
Luteolin, ≥98% (TLC), powder
Sigma-Aldrich
Ursolic acid, ≥90%
Sigma-Aldrich
Nervonic acid, ≥99% (capillary GC)
Sigma-Aldrich
Tricosanoic acid, ≥99% (capillary GC)
Sigma-Aldrich
Stearic acid, Grade I, ≥98.5% (capillary GC)