- Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells.
Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells.
FEBS letters (1995-05-08)
Y N Doza, A Cuenda, G M Thomas, P Cohen, A R Nebreda
PMID7750576
ABSTRACT
A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phosphorylation of both residues is required for the activation of this enzyme.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
p38α/SAPK2a Protein, active, 10 µg, Active, N-terminal GST tagged, recombinant, full length p38α/SAPK2a, for use in Kinase Assays.