Gel electrophoresis

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Components of Rehydration Solution

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Inserting Gels into Ettan™ DALT Electrophoresis Units

This protocol shows how to insert gels into Ettan™ DALT electrophoresis units.

Rehydration Solutions

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

Preparing for IEF

This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

Protein Visualization

Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.

DNA / Protein Electrophoresis and Troubleshooting Tables

Precipitation Procedures

This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.

Proteomic analysis of membrane proteins

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.

Auto2D® 2-D Gel Electrophoresis Device Frequently Asked Questions

Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.

How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System

Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.

Winning Westerns Technique Talk: Perfecting Western Blotting

In this webinar, Alli Lynch, Ph.D. reviews the basics of Western blotting, identifies common troubleshooting issues, and provides tips for Western blotting success.

Introduction to SDS-PAGE - Separation of Proteins Based on Size

Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.

Auto2D® Automated 2-D Gel Electrophoresis Device Protocol

The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.

Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.

ReadyBlue Protein Gel Staining Protocols

ReadyBlue™ Protein Gel Stain offers rapid Coomassie® staining for polyacrylamide gels with a simplified protocol.

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Troubleshooting 2-D DIGE Results

Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.

Water for Protein Electrophoresis & Western Blotting

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.

Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System

Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.

How to Run an mPAGE™ Protein Gel Using an Invitrogen XCell Surelock® Mini-Cell Electrophoresis System

Instructions for setting up an mPAGE™ SDS-PAGE precast protein gel for gel electrophoresis using an Invitrogen XCell Surelock® mini-cell.

Reproducibility with Biological Buffers

Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.

Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels

This page shows how to apply equilibrated Immobiline DryStrip gels

Formamide Molecular Biology Reagent

Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.

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