MABS449

Anti-Acylation Stimulating Protein Antibody, clone 4H3

clone 4H3, from mouse

• Synonym(s): Complement C3, C3adesArg, C3, PZP-like alpha-2-macroglobulin domain-containing protein 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4H3, monoclonal
• species reactivity: human
• technique(s): ELISA: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000055
• UniProt accession no.: P01024
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... C3(718)

Description

General description

The protein called Acylation stimulating protein (ASP) is a cleavage fragment protein from the Complement C3 gene. C3 plays a central role in the activation of the complement system. C3’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. ASP acts as an adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, and thus it helps regulate fat storage and triglyceride synthesis. ASP stimulates triglyceride synthesis via PLC, MAPK and AKT signaling pathways and ASP promotes the phosphorylation and internalization and recycling of Complement factor C5AR2. ASP is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. Increased levels of C3 and its cleavage product ASP are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

Immunogen

linear peptide corresponding to Human Acylation Stimulating Protein.

Application

ELISA Analysis: 7 µg/mL of this antibody detected ASP (PEG precipitated) in a sandwich ELISA format (Saleh, J,. et al, J. Lipid Res. 1998. 39: 884–891)

Research Category
Signaling

Metabolism

Research Sub Category
Insulin/Energy Signaling

Lipid Metabolism & Weight Regulation

This Anti-Acylation Stimulating Protein Antibody, clone 4H3 is validated for use in Western Blotting and ELISA for the detection of Acylation Stimulating Protein.

Quality

Evaluated by Western Blotting on purified Acylation Stimulating Protein from transfected HEK293 cell lysate

Western Blotting Analysis: 0.1 µg/mL of this antibody detected purified Acylation Stimulating Protein from transfected HEK293 cell.

Target description

~ 16/10 kDa observed, 10 kDa indicates native ASP, 16 kDa indicates HIS-Tag ASP. HIS-Tag was added for purification purposes. We have found no difference in Activity (measured by TGS, Fatty Acid uptake, etc) between rASP with/without the tag. Further, the ELISA was successful in measuring both.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1