For use in Equine sources, Protein G has a significantly higher affinity for IgG, as compared to protein A. Please see the link below to review a comparison table of Protein A to Protein G by species.
P8651
Protein A–Peroxidase from Staphylococcus aureus/horseradish
lyophilized powder
Synonym(s):
Peroxidase–Protein A
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0.5 MG
CHF 174.00
1 MG
CHF 338.00
2 MG
CHF 431.00
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0.5 MG
CHF 174.00
1 MG
CHF 338.00
2 MG
CHF 431.00
About This Item
Recommended Products
conjugate
peroxidase conjugate
Quality Level
100
200
form
lyophilized powder
technique(s)
direct ELISA: 1:80,000 using human IgG
storage temp.
−20°C
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General description
Protein A is a cell wall constituent of Staphylococcus aureus, with a molecular weight of 56kDa. It possesses five binding domains which interact with heavy chains of immunoglobulins at the Fc or Fab fragment.[1] In response to protein A, the complement system, interferon production, hypersensitivity reactions and cell-mediated cytotoxicity get activated. Horseradish peroxidase is a heme-containing enzyme.[2][3] In the presence of hydrogen peroxide, the enzyme catalyzes the oxidation of luminol.[4]
Specificity
Binds IgG only from most mammals, except rat, goat, sheep.
Application
Protein A–Peroxidase from Staphylococcus aureus/horseradish has been used in enzyme-linked immunosorbent assay (ELISA).[5]
Packaging
Package size based on protein content.
Physical form
Lyophilized powder containing sodium citrate buffer salt. Protein determined by A205.
Preparation Note
Protein A, extracellular, coupled to Peroxidase, Type VI, by a modification of the procedure of O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978), which favors low molecular weight conjugates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
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Hello, I would like to know if this conjugate serve for testing equine in a ELISA (Equus ferus caballus). Thank you
1 answer-
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What is the solution stability of Product P8651, Protein A Peroxidase?
1 answer-
The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20 °C.
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How do I prepare a stock solution of Product P8651, Protein A Peroxidase?
1 answer-
A stock solution of this product can be prepared in PBS (phosphate buffered saline) or other neutral buffer at 1 mg/mL.
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How do I use Product P8651, Protein A Peroxidase, as a my secondary in Western blot analysis?
1 answer-
For Western blotting, with chemiluminescent substrate, dilute 1:1000 to 1:10,000.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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I bought Product P8651, Protein A Peroxidase, to hide heavy and light chain in a Western blot. How does it do that?
1 answer-
P8651 will only recognize the native antibody incubated on a blot, not what's run in the gel. This should circumvent heavy and light chain detection.
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