WellRED Oligos

Designed for use with the CEQ/GeXP Genetic Analysis Systems, we are pleased to offer WellRED dye-labeled oligos under our license from Beckman Coulter, Inc. In addtion to their designed application, these labeled oligos may also be used in direct hybridization or PCR amplification. WellRED dye-labeled oligos are DNA oligonucleotides labeled with WellRED dyes, D2, D3 or D4, at the 5'end.

WellRED Specifiations

Yields	Cartridge: 2 and 4 OD HPLC: 5, 10, 50 and 100 OD
Purification	Cartridge, HPLC
Length	15 – 30 bases (inquire for longer lengths)
Backbone	Phosphodiester
Format	Supplied Dry
Modifications	D2-PA, D3-PA or D4-PA at the 5’end
Packaging	2 mL Opaque Tubes
Quality Controls	MALDI-TOF MS, Electrospray Ionization MS, OD by UV Spectroscopy
Technical Datasheet & Labels	Includes yield, Tm (melting temperature), MW, and µg/OD Duplicate set of labels with each order
Additional Services	Aliquoting into tubes MSQC traces available upon request
Shipping Schedule	Shipped within 4 to 5 business days

Benefits of WellRED Dye Chemistry

• WellRED oligos use cyanine-based fluorescent dyes with high extinction coefficients that absorb in the near-infrared region, thereby reducing background noise from biological materials, resulting in highly sensitive detection.
• The CEQ/GeXP Genetic Analysis Systems enable DNA samples to be kept in linear form during analysis. The CEQ/GeXP Genetic Analysis Systems' online denaturation process prevents any reformation of secondary structures, especially during a long run without any manual steps.
• WellRED dye chemistry and pre-heating enable the CEQ/GeXP Genetic Analysis Systems to resolve complex structures, where other systems fail.

Yields and Estimated Number of Assays

Yield (OD)	2	4	5	10
Purification	Cartridge	Cartridge	HPLC	HPLC
Approx. yield (nmols*)	10	20	25	50
Estimated assays (reactions)	1,000-2,000	2,000-4,000	2,500-5,000	5,000-10,000

*Estimate 1 OD = 5 nmols = 30 µg, for a 20 base oligo

Spectral Properties of WellRED Dyes

WellRED Oligo	Excitation maximum (nm)	Emission maximum (nm)	Molar extinction coefficient
D2-PA	750	770	170,000
D3-PA	685	706	224,000
D4-PA	650	670	203,000