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Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants.

Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants.

Nature plants (2015-09-01)

Erik Jonsson, Moé Yamada, Ronald D Vale, Gohta Goshima

ZUSAMMENFASSUNG

The molecular motors kinesin and dynein drive bidirectional motility along microtubules (MTs) in most eukaryotic cells. Land plants, however, are a notable exception, because they contain a large number of kinesins but lack cytoplasmic dynein, the foremost processive retrograde transporter. It remains unclear how plants achieve retrograde cargo transport without dynein. Here, we have analysed the motility of the six members of minus-end-directed kinesin-14 motors in the moss

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Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

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Chloroform, contains ethanol as stabilizer, meets analytical specification of BP, 99-99.4% (GC)

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Ethylenglykol-bis(2-aminoethylether)-N,N,N′,N′-Tetraessigsäure, ≥97.0%

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Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

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MOPS

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Ethylenglykol-bis(2-aminoethylether)-N,N,N′,N′-Tetraessigsäure, BioXtra, ≥97 .0%

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MOPS, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%