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A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.

Organic & biomolecular chemistry (2014-12-17)

S Bag, J-C Tseng, J Rochford

ZUSAMMENFASSUNG

Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investigated for the BODIPY-luminol dyad was found to be 64% resulting in a dual emissive response. Successful in cellulo biochemiluminescence via CRET was achieved in PMA activated splenocytes.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

Sigma-Aldrich

Tetrahydrofuran, inhibitor-free, suitable for HPLC, ≥99.9%

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Tetrahydrofuran, contains 200-400 ppm BHT as inhibitor, ACS reagent, ≥99.0%

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Phorbol-12-myristat-13-acetat, ≥99% (TLC), film or powder

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Natriumborhydrid, powder, ≥98.0%

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Natriumborhydrid, ReagentPlus^®, 99%

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p-Toluolsulfonsäure Monohydrat, ≥99% (calc. to H₂O free subst.)

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Tetrahydrofuran, ReagentPlus^®, ≥99.0%, contains 200-400 ppm BHT as inhibitor

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Triethylamin, BioUltra, ≥99.5% (GC)

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Tetrahydrofuran, contains 250 ppm BHT as inhibitor, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.9%

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Luminol, 97%

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Bor, ≥95% (boron), amorphous powder

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2,3-Dichlor-5,6-dicyano-p-benzochinon, 98%

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Natriumborhydrid, granular, 99.99% trace metals basis

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Natriumborhydrid, purum p.a., ≥96% (gas-volumetric)

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Phorbol-12-myristat-13-acetat, synthetic, ≥98.0% (TLC)

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Luminol, ≥97% (HPLC)

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Tetrahydrofuran, ACS reagent, ≥99.0%, contains 200-400 ppm BHT as inhibitor

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Triethylamin, ≥99.5%

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Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free

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Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%

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p-Toluolsulfonsäure Monohydrat, ACS reagent, ≥98.5%

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Triethylamin, ≥99%

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Natriumborhydrid -Lösung, ~12 wt. % in 14 M NaOH

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Natriumborhydrid, granular, 10-40 mesh, 98%

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Triethylamin, for amino acid analysis, ≥99.5% (GC)

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Triethylamin, ≥99.5%

Supelco

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